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In this experiment, as in most molecular biology techniques, a control must be used to ensure successful experimentation.
In molecular biology, procedures and technologies are continually being developed and older technologies abandoned.
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For example, before the advent of DNA gel electrophoresis (agarose or polyacrylamide), the size of DNA molecules was typically determined by rate sedimentation in sucrose gradients, a slow and labor-intensive technique requiring expensive instrumentation; prior to sucrose gradients, viscometry was used.
Aside from their historical interest, it is often worth knowing about older technology, as it is occasionally useful to solve another new problem for which the newer technique is inappropriate rapeutic delivery of nucleic acid polymers into a patient's cells as a drug to treat disease.
The target DNA is then washed and the labeled probes that didn't hybridize are removed.
The first therapeutic use of gene transfer as well as the first direct insertion of human DNA into the nuclear genome was performed by French Anderson in a trial starting in September 1990.Generally, efforts focused on administering a gene that causes a needed protein to be expressed.More recently, increased understanding of nuclease function has led to more direct DNA editing, using techniques such as zinc finger nucleases and CRISPR. The expressed nucleases then knock out and replace genes in the chromosome.Molecular biology concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, and proteins and their biosynthesis, as well as the regulation of these interactions.Writing in Nature in 1961, William Astbury described molecular biology as: "..so much a technique as an approach, an approach from the viewpoint of the so-called basic sciences with the leading idea of searching below the large-scale manifestations of classical biology for the corresponding molecular plan.